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Nucleic Acids Research, 1988, Vol. 16, No. 13 6223
© 1988

Articles

Cloning and nucleotide sequence of the gene encoding yeast ribosomal protein YS25

Katsuyuki Suzuki and Eiko Otaka

Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University 2-3 Kasumi I chome, Minamiku, Hiroshima 734, Japan

Accepted June 10, 1988.

Clones encoding ribosomal protein YS25* (1,2) were isolated from a genomic DNA library of Saccharomyces cerevisiae using synthetic oligonucleotides corresponding to the partial amino acid sequences of the protein. These sequence analyses suggest that the YS25 gene has an intron containing some features (underlined) in common with the other yeast ribosomal protein genes already determined, the ‘TACTAAC box’ of which is not strictly conserved. In complete agreement with the primary structure of protein YS25 previously reported (1), the amino acid sequence, deduced from the largest open reading frame, resulted in being split by the putative intron.


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