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Nucleic Acids Research, 1988, Vol. 16, No. 14 6531-6546
© 1988

Articles

RNA-primed intitiation sites of DNA replication in the origin region of bacteriophage {lambda} genome

Kin-ya Yoda, Hachiro Yasuda*, Xiao-Wang Jiang** and Tuneko Okazaki+

Department of Molecular Biology, School of Science, Nagoya University Chikusa-ku, Nagoya 464-01, Japan

+To whom correspondence should be addressed

Received March 8, 1988.

Using DNA molecules synthesized in the early stage of {lambda} phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the {lambda} phage genome containing the genetically defined replication origin (ori{lambda}). Sites in the 1-strand (the polarity of the 1-strand is 5' to 3' from the left to the right direction of the {lambda} phage genetic map) were distributed both inside and outside of the ori{lambda}, whereas the sites in the r-strand (the strand in the opposite polarity) were mainly distributed more than three hundred nueleotides apart from the ori{lambda} to the right. A CPuPu sequence was found at –12 to –10 region of transition sites of the r- and the 1-strands in the frequency of 80% and 70%, respectively, and over 60% of the CPuPu sequences were CAG. Properties of the transition sites are discussed in relation to the primer synthesis.

*Laboratory of Molecular Biology, Toyohaahi University of Technology, Tenpaku-3, cho, Toyohaahi, Aichi 440, Japan

**Institute of Health and Epidemic Prevention, Jiangau Province, P.R.China


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