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Nucleic Acids Research, 1988, Vol. 16, No. 15 7221-7231
© 1988

Articles

Preparation and characterization of blotinylated psoralen

Wilma A. Saffran1,2,*, John T. Welsh1, Robert M. Knobler2,+, Francis P. Gasparro3, Charles R. Cantor1 and Richard L. Edelson3

1Department of Genetics and Development Columbia University College of Physicians and Surgeons New York, NY 10032 2Department of Dermatology, Columbia University College of Physicians and Surgeons New York, NY 10032 3Deparment of Dermatology, Yale University School of Medicine New Haven, CT 06510, USA

Received June 21, 1988. Accepted July 6, 1988.

Biotinylated paoralen (BPaor), a paoralen derivative containing a biotin moiety attached via a long-chain, positively charged linker, has been syntheaized and its interactions with DNA and avidin have been studied. As do other psoralen derivatives, BPsor photoreacta with DNA to form interatrand crosalinka. The biotin binds to streptavidin after the reaction of BPaor with DNA, and this property has been used to measure low levels of BPsor. modified DNA by ELISA with streptavidin and biotinylated alkaline phoaphatase. In addition, BPaor retains the biological activity of psoralen, as shown by its ability to inhibit lymphocyte proliferation at a level of 10 ng/ml.

*Present address: Department of Chemistiy, Queens College-CUNY, Flushing, NY 11367, USA

+Present address: Department of Dermatology, University of Vienna, Vienna, Austria


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