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Nucleic Acids Research, 1988, Vol. 16, No. 15 7253-7267
© 1988

Articles

The 6-thioguanine/5-methyl-2-pyrimidinone base pair

Harry P. Rappaport

Biology Department, Temple University Philadelphia, PA 19122, USA

Received June 9, 1988. Accepted June 28, 1988.

As part of a program to determine the physical possibility of expanding the number of types of base pairs in DNA, the pairing stabilities of the analog bases 6-thioguanine (Ga) and 5-nethyl-2-pyrimidinone (Tm) in oligodeoxynucleotides were measured. Procedures were developed to synthesize oligodeoxynucleotides with the analog bases. The sequences of the synthesized oligomers were T-C-G-A-C-G-G-X-Y-C-C-G. An enzymatic procedure was developed to measure relative association constants of oligomer pairs with the self complementary reference oligomer, X=A and Y=T, K(T/A)=K The results were K(C/G)=(5±.5)K, K(Tm/Gs)=K/(1±.5), K(T/G)=K/(9±3), K(Tb/G)=K/(25±5), K(C/GH) <K/30, K(TH/A) <K/40, K(T/Gs) <K40, K(C/A)<K/40. The results with the standard bases are consistent with other methods of measurement. The stability of the base pair Gs/TH is approximately the same as the standard base pair A/T.


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