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Nucleic Acids Research, 1988, Vol. 16, No. 16 7833-7842
© 1988

Articles

Novobiocin induces accumulation of a single strand of plasmid pGRB-1 in the archaebacterium Halobacterium GRB

Mouldy Sioud, Giuseppe Baldacci, Patrick Foeterre+ and Anne-Marie de Recondo

Laboratoire de Biologic Moléculaire de la Replication ER 272, CNRS-IRSC, 7 rue Guy Móquet, BP no. 8, 94 802 Vilejuif Célex, France

+Present address: Institut de Microbiologie, Bätiment 409, 91405 Orsay, France

Received June 14, 1988. Accepted July 20, 1988.

Treatment of Halobacterium GRB cells with the DNA topoisomerase II inhibitor novobiocin induces the accumulation of a circular single-stranded DNA form of the plasmid pGRB-1. This form corresponds to the transcribed strand of pGRB-1. A tiny amount of this form is detectable in untreated cells. The induction of single-stranded pGRB-1 molecules by novobiocin is abolished when cells are pretreated with aphidicolin or anisomycin, which inhibit halobacterial DNA replication and protein synthesis, respectively. These results suggest that the single-stranded form of pGRB-1 is generated in the course of plasmid replication.


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