Skip Navigation

This Article
Right arrow Print PDF (507K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Taidi-Laskowski, B.
Right arrow Articles by Grumet, F.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taidi-Laskowski, B.
Right arrow Articles by Grumet, F.C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 16 8157-8169
© 1988

Articles

Use of RecA protein to enrich for homologous genes in a genomic library

Behnaz Taidi-Laskowski, Dolly Tyan1, Saul M. Honigberg2, Charles R. Radding2 and F.Carl Grumet*,

Department of Pathology, Stanford University School of Medicine Stanford, CA 94305 1University of California Los Angeles, CA 2Department of Human Genetics, Yale University School of New Haven, CT, USA

*To whom correspondence should be addressed

Received April 25, 1988. Revised July 22, 1988. Accepted July 22, 1988.

RecA protein-coated probe has been utilized to enrich genomic digests for desired genes in order to facilitate cloning from genomic libraries. Using a previously cloned HLA-B27 gene as the recA-coated enrichment probe, we obtained a mean 108x in crease in the ratio of specific to nonspecific plaques in lambda libraries screened for B27 variant alleles of estimated 99% homology to the probe. Class I genes of lesser homology were less enriched: 6.7x for non-B27 genes of estimated >95% homology and 3.7x for other Class I genes of >80% homology. Loss of genomic DNA during the enrichment procedure can, however, re strict application of this technique whenever starting genomic DNA is very limited. Nevertheless, the impressive reduction in cloning effort and material makes recA enrichment a useful new tool for cloning homologous genes from genomic DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. J. Ferrin and R. D. Camerini-Otero
Sequence-specific ligation of DNA using RecA protein
PNAS, March 3, 1998; 95(5): 2152 - 2157.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.