Skip Navigation

This Article
Right arrow Print PDF (2251K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Samuelson, L. C.
Right arrow Articles by Meisler, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samuelson, L. C.
Right arrow Articles by Meisler, M. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 17 8261-8276
© 1988

Articles

Expression of the human amylase genes: recent origin of a salivary amylase promoter from an actin pseudogene

Linda C. Samuelson, Karin Wiebauer1, Deborah L. Gumucio and Miriam H. Meisler*

Department of Human Genetics, University of Michigan Ann Arbor, MI 48109-0618, USA 1Friedrich Miescher Institut Postfach 2543, CH-4002 Basel, Switzerland

*To whom correspondence should be addressed

Received July 5, 1988. Accepted July 29, 1988.

The human genes encoding salivary amylase (AMY1) and pancreatic amylase (AMY2) are nearly identical in structure and sequence. We have used ribonuclease protection studies to identify the functional gene copies in this multigene family. Riboprobes derived from each gene were hybridized to RNA from human pancreas, parotid and liver. The sizes of the protected fragments demonstrated that both pancreatic genes are expressed in pancreas. One of the pancreatic genes, AMY2B, is also transcribed at a low level in liver, but not from the promoter used in pancreas. AMY1 transcripts were detected in parotid, but not in pancreas or liver. Unexpected fragments protected by liver RNA led to the discovery that the 5' regions of the five human amylase genes contain a processed {gamma}-actin pseudogene. The promoter and start site for transcription of AMY1 are recently derived from the 3' untranslated region of {gamma}-actin. In addition, insertion of an endogenous retrovirus has interrupted the {gamma}-actin pseudogene in four of the five amylase genes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-L. Da Lage, E. Renard, F. Chartois, F. Lemeunier, and M.-L. Cariou
Amyrel, a paralogous gene of the amylase gene family in Drosophila melanogaster and the Sophophora subgenus
PNAS, June 9, 1998; 95(12): 6848 - 6853.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
V Giguere, M Tini, G Flock, E Ong, R M Evans, and G Otulakowski
Isoform-specific amino-terminal domains dictate DNA-binding properties of ROR alpha, a novel family of orphan hormone nuclear receptors.
Genes & Dev., March 1, 1994; 8(5): 538 - 553.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
C N Ting, M P Rosenberg, C M Snow, L C Samuelson, and M H Meisler
Endogenous retroviral sequences are required for tissue-specific expression of a human salivary amylase gene.
Genes & Dev., August 1, 1992; 6(8): 1457 - 1465.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.