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Nucleic Acids Research, 1988, Vol. 16, No. 17 8323-8336
© 1988

Articles

Physical mapping of the Mycopiasma pneumoniae genome

Rainer Wenzel and Richard Herrmann

Microbiology, University of Heidelberg Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG

Received June 27, 1988. Revised August 5, 1988. Accepted August 5, 1988.

In order to study the genome organization of Mycoplasma pneumoniae a cosmid library of M.pneumoniae DNA was established using a newly designed cosmid vector (pcosRW2). From this library 32 overlapping clones were isolated covering a contiguous 720 kbp DNA segment representing about 90% of the genome assuming a genome size of about 800 kbp.


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