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Nucleic Acids Research, 1988, Vol. 16, No. 17 8411-8431
© 1988

Articles

Recognition of prokaryotic transcription terminators by spinach chloroplast RNA polymerase

Liang-Jwu Chen1 and Emil M. Orozco, Jr1,2,*

1Department of Agronomy, University of Illinois Urbana, IL 61801, USA 2Agricultural Research Service, United States Department of Agriculture 1102 South Goodwin Avenue, Urbana, IL 61801, USA

*To whom correspondence should be addressed

Received June 8, 1988. Revised August 11, 1988. Accepted August 11, 1988.

To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, rrnB, rrnC and gene 32 terminators. The most efficient transcription termination was observed for the threonine attenuator. For each of the prokaryotic terminators, the chloroplast enzyme ceased transcription at essentially the same position as the E. coli RNA polymerase. These data indicate that the transcription termination process in chloroplasts has some features in common with the mechanism used in prokaryotes.


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[Abstract] [PDF]


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