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Nucleic Acids Research, 1988, Vol. 16, No. 17 8555-8569
© 1988

Articles

A structure of potentially active and inactive genes of chicken erythrocyte chromatin upon decondensation

Alexander N. Kukushkin, Svetlana B. Svetlikova and Valery A. Pospelov

Institute of Cytology, USSR Academy of Sciences Leningrad, 194064, USSR

Received April 1, 1988. Revised August 4, 1988. Accepted August 4, 1988.

In the presence of 3 mM MgC12 DNase I cleavage of bulk, globin and ovalbumin gene chromatrn in chicken erythrocyte nuclei generates fragments which are multiples of a double-nucleosome repeat. However, in addition to the dinucleosomal periodicity B-globin gene chromatin was fragmented into multiples of a 100 b.p. interval which is characteristic for partially unfolded chromatin. This distinction correlates with higher sensitivity of B-globin domain to DNase I and DNase II as compared to the inactive ovalbumin gene. At 0.7 mM MgCl2 where these DNases fragment bulk chromatin into series of fragments with a 100 b.p. interval, the difference in digestibility of the investigated genes is dramatically decreased. When chromatin has been decondensed by incubation of nuclei in 10 mM Trie-buffer, DNase II generates a typical nucleosomal repeat, and the differential nuclease sensitivity of the analyzed genes is not observed. The data suggest that higher nuclease sensitivity of potentially active genes is due to irregularities in higher order chromatin structure.


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