Nucleic Acids Research, 1988, Vol. 16, No. 18 8835-8846
© 1988
Articles |
5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene
Department of Medicine, Howard Hughes Medical Institute, University of Chicago Chicago, IL 60637, USA
Received June 9, 1988. Revised August 23, 1988. Accepted August 23, 1988.
Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and consists of 2 exons and one 700 bp intron. S1 nuclease protection and primer extension analysis were used to define the transcription initiation site. "TATA" and "CCAAT" sequences were located at nucleotides –26 and –337 respectively. In addition, there exist five elements whose sequence is nearly identical to the inner core 10 nucleotide region (CCATATTAGG) of the c-fos serum response element, four Spl consensus sequences, two AP1 target sequence analogs, and two potential cAMP response elements. These results will ultimately lead to a detailed definition of the intracellular events regulating Egr-1 expression.
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