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Nucleic Acids Research, 1988, Vol. 16, No. 18 8847-8868
© 1988

Articles

Foreign transcriptional enhancers in yeast. I. Interactions of papovavirus transcriptional enhancers and a quiescent pseudopromoter on supercoiled plasmids

Maria Ciaramella, Margherita Sacco and John F. Pulitzer

International Institute of Genetics and Biophysics (CNR) via G.Marconi 10, Naples 80125, Italy

Received June 9, 1988. Revised July 26, 1988. Accepted July 26, 1988.

We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudo-promoter. We investigate the function in yeast of foreign control elements, the polyomavirus enhancer and some of its derivatives, inserted in either orientation at the 3' or 5' ends of the reporter gene. The polyoma enhancer (and, although less thoroughly studied, the SV40 enhancer) will strongly activate transcription from latent start sites within the pseudo-promoter sequence. The rules we draw for the polyoma enhancer effect in yeast are, with a few interesting exceptions, remarkably similar to those discovered by experimentation in mammalian cells.


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