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Nucleic Acids Research, 1988, Vol. 16, No. 18 8977-8997
© 1988

Articles

Deletions in the SV40 late polyadenylation region downstream of the AATAAA mediate similar effects on expression in various mammalian cell lines

Edward R. Gimmi1,2, Kenneth J. Soprano1, Martin Rosenberg2 and Mitchell E. Reff1,2

1Department of Microbiology and Immunology, Temple University School of Medicine Philadelphia, PA 19140 2Department of Molecular Genetics, Smith, Kline & French Laboratories 709 Swedeland Road, King of Prussia, PA 19406, USA

Received March 15, 1988. Revised August 11, 1988. Accepted August 11, 1988.

A series of deletions in the SV40 late polyadenylation region was assayed by transient expression in a hamster fibroblast cell line. Because of differences in expression data between our results and the published results of another laboratory using a similar set of deletions introduced into a monkey kidney cell line, we studied our deletions in cells of different tissue-types and species (1). Deletion of the SV40 late polyadenylation region to 49 nucleotides downstream of the hexanucleotide AATAAA showed a small effect on gene expression, while further truncation of the region to 6 nucleotides downstream of the AATAAA showed an 85% drop in marker enzyme activity, protein levels and steady-state message levels. Another deletion in the same region, from base pair 10 to 15 past the AATAAA, which removes the wild-type site of RNA cleavage, showed a 50% drop in marker gene expression. The effects of these mutants on gene expression were similar in all of the cell lines tested and agree with other studies that DNA downstream of the AATAAA plays a role in efficient gene expression.


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