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Nucleic Acids Research, 1988, Vol. 16, No. 19 9267-9283
© 1988

Articles

Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants

Caroline Dean1, Jonathan Jones2, Mitchell Favreau, Pamela Dunsmuir* and John Bedbrook

Advanced Genetic Sciences Inc. 6701 San Pablo Ave, Oakland, CA 94608, USA

*To whom correspondence should be addressed

Received January 1, 1988. Accepted September 6, 1988.

The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco plants which had been standardized physiologically as much as possible. The presence of adjacent pUC plasmid sequences did not affect the expression of the SSU301 gene. In an attempt to reduce the between-transformant variability in expression, the SSU301 gene was introduced into tobacco surrounded by 10kb of 5' and 13kb of 3' DNA sequences which normally flank SSU301 in petunia. The longer flanking regions did not reduce the between-transformant variability of SSU301 gene expression.

1Present addresses: IPSR, Colney Lane, Norwich, NR4 7UH, UK

2John Innes Institute, Colney Lane, Norwich, NR4 7UH, UK


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