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Nucleic Acids Research, 1988, Vol. 16, No. 2 685-701
© 1988

Articles

Comparative calorimetric studies on the dynamic conformation of plant 5S rRNA. 1. Thermal unfolding pattern of lupin seeds and wheat germ 5S rRNAs, also in the presence of magnesium and sperminhim cations

J. Barciszewski, M.D. Bratek-Wiewiórowska, P. Górnicki, M. Naskret-Barciszewska, M. Wiewiórowski, A. Zielenkiewicz1 and W. Zielenkiewicz1

Institute of Bioorganic Chemistry, Polish Academy of Sciences Noskowskiego 12/14, 61–704 Poznan 1Institute of Physical Chemistry, Polish Academy of Sciences Kasprzaka 44/52, 01–224 Warsaw, Poland

Received October 22, 1987. Revised December 3, 1987. Accepted December 3, 1987.

An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher order structures. Interesting effects induced by magnesium cation, perprotonated and permethylated sperminium tetracations are discussed. It is suggested that the difference in the stabilizing effect of the three cations results from different mode of their interactions with RNA. "Pure" electrostatic interactions expected for permethylated tetracations are rather weak due to the steric hindrance around each positively charged nitrogen atom. Electrostatic interactions of the other two cations are significantly enhanced by coordination bonding for magnesium and by hydrogen bonding for protonated sperminium cation.


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