Skip Navigation

This Article
Right arrow Print PDF (452K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (47)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tajmir-Riahi, H.A.
Right arrow Articles by Savoie, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tajmir-Riahi, H.A.
Right arrow Articles by Savoie, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 2 751-762
© 1988

Articles

A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes

H.A. Tajmir-Riahi, M. Langlais and R. Savoie

Department of Chemistry and CRAM, Laval University Quebec, QC G1K 7P4, Canada

Received July 20, 1987. Revised December 7, 1987. Accepted December 7, 1987.

The Interaction of calf-thymus DNA with Cu(II) and Pb(II) ions has been Investigated In H20 and D,0 solutions at physiological pH, using laser Raman spectroscopy. The results confirm the destabilizing effect of Cu2+ ions, which are shown to bind strongly to the guanlne and cytldine bases, perturbing the A-T base pairs and disrupting the double-helical struoture of DNA, whose conformation is markedly altered by these Interactions. Earlier claims that Pb2+ ions destabilize DNA are not supported by the present study. These ions are found to interact only weakly with the nucleic bases, binding to the N7 position of the guanlne bases and also interacting with the A-T pairs. Both types of Ions are found to Interact with the charged phosphate groups of DNA, although these sites are preferred over the nucleic bases by Pb2+ ions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.