Skip Navigation

This Article
Right arrow Print PDF (1507K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kupiec, J.-J.
Right arrow Articles by Emanoil-Ravier, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kupiec, J.-J.
Right arrow Articles by Emanoil-Ravier, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 20 9557-9565
© 1988

Articles

Evidence for a gapped linear duplex DNA intermediate in the replicative cycle of human and simian spumavirases

Jean-Jacques Kupiec, Joëlle Tobaly-Tapiero, Martine Canivet, Maud Santillana-Hayat, Rolf M. FlÜgel1, Jorge Périès and Rodica Emanoil-Ravier

UPR 0049 CNRS ‘Rétrovirus et Rétrotransposons des Vertébrés’, HÔpital Saint-Louis 1 Avenue Claude Vellefaux, 75010 Paris, France 1Institute of Virus Research, German Cancer Research Center 6900 Heidelberg, FRG

Received July 15, 1988. Accepted September 16, 1988.

Two forms of linear DNAs have been found in simian (SFV1) and human (HSRV) spumaviruses: a linear duplex unsensitive to nuclease S1 and a sensitive structure with a single-stranded gap. Two nuclease S1 sensitive sites, mapping at the same position for both viruses, have been identified in the gapped structure. Using different molecular subgenomic clones of HSRV as probes in Southern blot analysis, one S1 site was localized in the 3'LTR and the other near the middle of the molecule at about 6.5 kbp from the 5' end of the viral genome. The latter site was shown to correspond to a single stranded region within the linear duplex DNA. Nucleotide sequence analysis revealed that the polypurine tract (PPT) usually found at the 5' boundary of the 3'LTR of retroviruses, is duplicated in HSRV at the 3' end of the pol gene, near the gap. This suggests that the synthesis of plus strand DNA is discontinous, generating the gap.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
R. Suspene, C. Rusniok, J.-P. Vartanian, and S. Wain-Hobson
Twin gradients in APOBEC3 edited HIV-1 DNA reflect the dynamics of lentiviral replication
Nucleic Acids Res., October 18, 2006; 34(17): 4677 - 4684.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Wurtzer, A. Goubard, F. Mammano, S. Saragosti, D. Lecossier, A. J. Hance, and F. Clavel
Functional Central Polypurine Tract Provides Downstream Protection of the Human Immunodeficiency Virus Type 1 Genome from Editing by APOBEC3G and APOBEC3B.
J. Virol., April 1, 2006; 80(7): 3679 - 3683.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Peters, T. Wiktorowicz, M. Heinkelein, and A. Rethwilm
RNA and Protein Requirements for Incorporation of the Pol Protein into Foamy Virus Particles
J. Virol., June 1, 2005; 79(11): 7005 - 7013.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Whitwam, M. Peretz, and E. Poeschla
Identification of a Central DNA Flap in Feline Immunodeficiency Virus
J. Virol., October 1, 2001; 75(19): 9407 - 9414.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Tobaly-Tapiero, P. Bittoun, M. Neves, M.-C. Guillemin, C.-H. Lecellier, F. Puvion-Dutilleul, B. Gicquel, S. Zientara, M.-L. Giron, H. de Thé, et al.
Isolation and Characterization of an Equine Foamy Virus
J. Virol., May 1, 2000; 74(9): 4064 - 4073.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
L. A. LaPierre, D. L. Holzschu, P. R. Bowser, and J. W. Casey
Sequence and Transcriptional Analyses of the Fish Retroviruses Walleye Epidermal Hyperplasia Virus Types 1 and 2: Evidence for a Gene Duplication
J. Virol., November 1, 1999; 73(11): 9393 - 9403.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. L. Linial
Foamy Viruses Are Unconventional Retroviruses
J. Virol., March 1, 1999; 73(3): 1747 - 1755.
[Full Text]

Home page
 J. Virol.Home page
S. F. Yu, M. D. Sullivan, and M. L. Linial
Evidence that the Human Foamy Virus Genome Is DNA
J. Virol., February 1, 1999; 73(2): 1565 - 1572.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
R. J. Noad, N. S. Al-Kaff, D. S. Turner, and S. N. Covey
Analysis of Polypurine Tract-associated DNA Plus-strand Priming in Vivo Utilizing a Plant Pararetroviral Vector Carrying Redundant Ectopic Priming Elements
J. Biol. Chem., December 4, 1998; 273(49): 32568 - 32575.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Heinkelein, M. Schmidt, N. Fischer, A. Moebes, D. Lindemann, J. Enssle, and A. Rethwilm
Characterization of a cis-Acting Sequence in the pol Region Required To Transfer Human Foamy Virus Vectors
J. Virol., August 1, 1998; 72(8): 6307 - 6314.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
O. Erlwein, P. D. Bieniasz, and M. O. McClure
Sequences in pol Are Required for Transfer of Human Foamy Virus-Based Vectors
J. Virol., July 1, 1998; 72(7): 5510 - 5516.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.