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Nucleic Acids Research, 1988, Vol. 16, No. 21 10037-10052
© 1988

MOLECULAR BIOLOGY

Promoter analysis of myosin alkali light chain genes expressed in mouse striated muscle

Arlette Cohen, Paul J.R. Barton*, Benoît Robert, Ian Garner, Serge Alonso and Margaret E. Buckingham

Département de Biologie Moléculaire, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris cedex 15, France

* To whom correspondence should be addressed

Received August 15, 1988. Accepted October 6, 1988.

There are three principal myosin alkali light chain (MLC) genes expressed in mouse striated muscle. The skeletal muscle gene MLC1F/MLC3F, the ventricular muscle / slow skeletal muscle aene MLC1V/(MLC1S), and the atrlal muscle / foetal striated muscle gene MLC1A(MLC1emb). MLC1V and MLC1A are expressed in both cardiac and skeletal muscle, and we show here that these genes use a single site of initiation of transcription, and therefore the same proximal promoter region, in both muscle types, and in myogenic cell lines in culture. We have previously shown that for the MLC1F/MLC3F gene, 1200bp of upstream sequence from the MLC1F promoter is sufficient to allow tissue specific and developmentally regulated expression. We have therefore isolated, characterised, and sequenced over 1200bp upstream of each of the three MLC genes in order to look for elements which may be involved in their regulation. Detailed comparison of their promoter sequences, as well as those of the cardiac and skeletal muscle {alpha}-actin genes, reveals a number of common elements. Amoung these is an "MLC-sequence" (CCTTTTATAG) common to all MLC genes, including those of chick and rat, and a "cardiac sequence" common to the mouse MLC1A, MLC1V and {alpha}-cardiac actin genes expressed in the heart.


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