Skip Navigation

This Article
Right arrow Print PDF (1818K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Shpakovski, G. V.
Right arrow Articles by Berlin, Yu. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shpakovski, G. V.
Right arrow Articles by Berlin, Yu. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 21 10199-10212
© 1988

MOLECULAR BIOLOGY

Structural bases of a long-stretched deletion: completing the {lambda}plac5 DNA primary structure§

G. V. Shpakovski, A. A. Akhrem and Yu. A. Berlin1

Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences Minsk 220023, USSR 1Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences Mascow 117811, USSR

Received July 8, 1988. Accepted October 8, 1988.

In studying molecular mechanisms of specialised transduction. the lac1 (E. coli)-Ea47({lambda}) DNA junction in transducing bacteriophage {lambda}plac5 has been structural1y elucidated, thus yielding the complete sequence of {lambda}plac5 DNA including the lac5 substitution, a well-known segment of lambdoid vectors. The {lambda}phac5 DNA is shown to consist of 19368 bp ({lambda} left arm) + 3924 bp {lambda}plac5 substitution)+25353 bp ({lambda} right arm), totally amounting to 48645 bp. The presence of the phage pbL promoter near to the right end of the lac5 insert is shown. The lacl gene distal end in {lambda}plac5 proved to be much longer than it was oostulated earlier. codinq for 224 C-terminal amino acid residues of lac repressor. Both the recombination studied in this paper and the earlier studied abnormal prophage excision (2,3) occur near to Chi-like structures (chi*Lac1 and chi*lom, respectively). On thebasis of the data obtained, a key role of the E. coli RecBCD system and Chi-like sequences in the formation of deletions in bacterial cells is suggested.

§Part of this work was published in a Letter to the Editor (1).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.