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Nucleic Acids Research, 1988, Vol. 16, No. 22 10469-10476
© 1988

MOLECULAR BIOLOGY

A novel nuclear transcription system which responds correctly to cloned estrogen receptor

Andrew Thorburn and John Knowland*

Department of Biochemistry South Parks Road, Oxford, 0X1 3QU, UK

*To whom correspondence should be addressed

Received September 2, 1988. Revised October 28, 1988. Accepted October 28, 1988.

We describe here a novel but simple nuclear transcription system in which nuclei make RNA in an lsotonlc buffer for a long time and respond to a cloned external factor by accurately initiating new transcription.


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