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Nucleic Acids Research, 1988, Vol. 16, No. 22 10733-10750
© 1988

MOLECULAR BIOLOGY

Effects of palindromes on in vivo DNA replication and mutagenesls in bacteriopnage FE-X174 RF DNA

Uwe R. Müller+, Barbara K. Perkins and W.Lee Williams

Department of Microbiology and Immunology, East Carolina University School of Medicine Greenville, NC 27858-4354, USA

+To whom correspondence should be addressed at

Received June 16, 1988. Revised October 17, 1988. Accepted October 17, 1988.

Bacteriophage FEX174 mutants with insertions of palindromic DNA sequences are rapidly outgrown by competing wild-type phage (Müller & Turnage, J. Mol. Biol. 189: 285). The basis for this defect was investigated and found to be due to an exclusion event early in the infectious cycle, in which phage genomes with palindromic inserts were preferentially excluded by wt phage. In addition, we have obtained further evidence for a palindrome induced genetic instability. Both defects are dependent on palindrome size and sequence, consistent with a model which involves formation of cruciforms, or cruciform-like structures. We propose that formation of unusual DNA secondary structures reduces the effectiveness of replicative form (RF) DNA to interact with limiting replication factors or membrane binding sites, possibly because of interaction with the host recombination system.


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