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Nucleic Acids Research, 1988, Vol. 16, No. 3 1153-1167
© 1988

Articles

Preliminary spectroscopic characterization of a synthetic DNA oligomer containing a B–Z junction at high salt

Richard D. Sheardy

Department of Chemistry, Pennsylvania State University Hazleton Campus, Hazleton, PA 18201, USA

Received September 3, 1987. Accepted December 3, 1987.

It is well known that the local conformation of a segment of DNA is dependent upon both the sequence of the segnent and the conditions under which the DNA is prepared. In extreme cases, the DNA may contain regions of both right and left-handed conformations, mandating the existence of a conformational junction between the two. These B–Z junctions have been observed in plasmids but, to date, no model systems have been characterized to determine the molecular nature of these junctions. Preliminary CD, UV, and NNR studies on such a model are presented here. A 16 base pair oligonucleotide, containing a potential B–Z junction, has been synthesized and characterized by the above techniques. The results suggest that this molecule contains both right and left-handed conformations under condition of high salt, and thus a B–Z junction.


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J. M. Ferreira and R. D. Sheardy
Enthalpy of the B-to-Z Conformational Transition of a DNA Oligonucleotide Determined by Isothermal Titration Calorimetry
Biophys. J., November 1, 2006; 91(9): 3383 - 3389.
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