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Nucleic Acids Research, 1988, Vol. 16, No. 3 1169-1180
© 1988

Articles

An ARS element from Drosophila melanogaster telomeres contains the yeast ARS core and bent replication enhancer

Alexander I Gragerov, Olga N. Danilevskaya, Svetlana A. Didichenko and Ekaternia N. Kaverina

Institute of Molecular Genetics, USSR Academy of Sciences Kurchatov Sq. 46, Moscow 123182, USSR

Received August 28, 1987. Revised January 11, 1988. Accepted January 11, 1988.

We have sequenced the 0.7-kb-long fragment of Drosophila DNA which ensures the autonomous replication of plasmids in yeast. Deletion mapping has shown the ARS element to consist of at least two domains: the core having the consensus 11-bp sequence TAAATATAAAT and the enhancer which is no more than 90 bp long and is located at the 3'-end of the A-rich core strand. Neither domaine per se ensures plasmid replication in yeast. A comparison of the enhancer sequence with the sequences of 14 different ARS elements failed to reveal significant homology areas. Most probably the ARS flanks that are adjacent to the core and act as enhancer do not carry any consensus. They may determine a peculiar structural feature of DNA (for example bends) which are necessary for the protein - ARS interaction.


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