Nucleic Acids Research, 1988, Vol. 16, No. 4 1563-1575
© 1988
Articles |
McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning
New England Biolabs 32 Tozer Rd, Beverly, MA 01915, USA 1Department of Molecular Biology, University of Edinburgh King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK 2Department of Molecular Genetics and Cellular Biology, University of Chicago Chicago, IL 60637 3Department of Microbiology, Medical College of Ohio Toledo, OH 43699, USA 3Department of Neurology, School of Medicine, University of California San Francisco, CA 94143, USA 5Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research Mill Hill, London NW7 1AA, UK 6MSU-DOE Plant Research Laboratory, Michigan State University East Lansing, MI 48824 7Cold Spring Harbor Laboratory Box 100, Cold Spring Harbor, NY 11724, USA
Received November 16, 1987. Revised December 29, 1987. Accepted December 29, 1987.
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. The McrA and B pheno-types of a few strains have been reported previously (1–4). The Mcr phenotypes of 94 strains, primarily derived from E. coli K12, are tabulated here. We briefly review some evidence suggesting that McrB restriction of mouse-modified DNA does occur in vivo and does in fact interfere with cloning of specific mouse sequences.
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