Skip Navigation

This Article
Right arrow Print PDF (2353K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rooney, R. J.
Right arrow Articles by Harding, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rooney, R. J.
Right arrow Articles by Harding, J. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 6 2509-2521
© 1988

Articles

Transcriptional activity and factor binding are stimulated by separate and distinct sequences in the 5' flanking region of a mouse tRNAAsp gene

Robert J. Rooney1 and John D. Harding2,*

Department of Biological Sciences, Columbia University New York, NY 10027, USA

*To whom correspondance should be address

Received December 4, 1987. Revised February 18, 1988. Accepted February 18, 1988.

The transcriptional properties of two cloned mouse tRNAASP genes were examined in vitro. The tRNA2Asp gene displays a five fold greater transcriptional activity than the tRNA1Asp gene and a greater ability to form stable complexes with transcription factors. Transcription of a hybrid gene with swapped 5' flanking sequences and of 5' flanking region deletion mutants demonstrates that the differential transcription of the genes results from stimulatory sequences in the 5' flanking region of the tRNA2Asp gene. Distal sequences including those between positions -53 and -31 stimulate transcription but do not affect factor binding. Proximal sequences between positions -9 and-i enhance factor binding. Thus, binding of transcription factors and later steps required for transcription can be modulated by separate and distinct 5' flanking sequence motifs in eukaryotic tRNA genes.

1Present Address: Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710

2Present Address: Bethesda Research Laboratories, 8717 Grovemont Circle, Gaithersburg, MD 20877.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Sharma and K. P. Gopinathan
Transcriptional Silencing of a tRNA1Gly Copy from within a Multigene Family Is Modulated by Distal cis Elements
J. Biol. Chem., November 8, 1996; 271(45): 28146 - 28153.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. F. Reynolds and W. F. Reynolds
Developmental Stage-specific Regulation of Xenopus tRNA Genes by an Upstream Promoter Element
J. Biol. Chem., May 5, 1995; 270(18): 10703 - 10710.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.