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Nucleic Acids Research, 1988, Vol. 16, No. 7 3053-3060
© 1988

Articles

Bcefl, a new type IIs restriction endonuclease

Pál Venetianer and András Orosz

Institute of Biochemistry, Biological Research Center PO Box 521, H-6701, Szeged, Hungary

Received December 4, 1987. Revised February 10, 1988. Accepted February 10, 1988.

A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a ±1 basepair uncertainity. Thus the cleavage and recognition site is as shown below:

ACGGC(N)11-13

TGCCG(N)12-14


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