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Nucleic Acids Research, 1988, Vol. 16, No. 7 3075-3089
© 1988

Articles

Cloning and characterization of a gene from Rhizobium melilotii 2011 coding for ribosomal protein S1

Joachim Schnier1,*, Sabine Thamm, Rudi Lurz, Atta Hussain2, Gabriele Faist3 and Beate Dobrinski

Max-Planck-Institut für Molekulare Genetik D-1000 Berlin 33 (Dahlem), Ihnestrasse 73, FRG

*To whom correspondence should be addressed

Received November 11, 1987. Accepted March 2, 1988.

A 7 kb chromosomal DNA fragment from R. melilotii was cloned, which complemented temperature-sensitivity of an E. coli amber mutant in rpsA. the gene for ribosomal protein S1 (ES1). From complementation and maxicell analysis a 58 kd protein was identifled as the homolog of protein S1 (RS1). DNA sequence analysis of the R. melilotii rpsA gene identified a protein of 568 amino acids, which showed 47% identical amino acid homology to protein S1 from E. coli The RS1 protein lacked the two Cys residues which had been reported to play an important role for the func tion of ES1. Two repeats containing Shine-Dalgarno sequences were identified upstream of the structural gene. Binding studies with RNA polymerase from E. coil and Pseudomonas putida located one RNA-polymerase binding site close to the RS1 gene and another one several hundred basepairs upstream. One possible promoter was also identified by DNA sequence comparison with the corresponding E. coli promoter.

Present address: 1Department of Microbiology and Immunology, University of California, Berkeley, CA 94720, USA

Present address: 2Umea University, Unit for Applied Cell and Molecular Biology, Umea S-90187, Sweden

Present address: 3Serono, Taunusstrasse 24, D-1000 Berlin 41, FRG


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