Nucleic Acids Research, 1988, Vol. 16, No. 8 3175-3194
© 1988
Articles |
Two genes encode related cytoplasmic elongation factors 1
(EF-1) in Drosophila melanogaster with continuous and stage specific expression
Zentrum für Molekulare Biologie Heidelberg (ZMBH), Universität Heidelberg Im Neuenheimer Feld 282, D 6900 Heidelberg, FRG 1Biozentrum, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
Received February 4, 1988. Accepted March 18, 1988.
We have characterized two previously cloned genes, F1 and F2 (1) that code for elongation factor EF - 1
of Drosophila melanoaaster. Genomic Southern blot hybridization revealed that they are the only gene copies present. We isolated cDNA clones of both transcripts from embryonal and pupal stage of development that cover the entire transcription unit. The 5' ends of both genes have been determined by primer extension and for F1 also by RNA sequencing. These start sites have been shown to be used consistently during development. Comparison of cDNA and genomic sequences revealed that EF - 1,F1 consists of two and EF - 1,F2 of five exons. The two described elongation factor genes exhibit several regions of strong sequence conservation when compared to five recently cloned eucaryotic elongation factors.
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