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Nucleic Acids Research, 1988, Vol. 16, No. 9 3693-3704
© 1988

Articles

An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter

Ralph Mocikat, Ger J.M. Pruijn1, Peter C. van der Vliet1 and Hans G. Zachau

Institut f{euro}r Physiologische Chemie, Physikalische Biochemie and Zellbiologie der Universität M{euro}nchen FRG 1Laboratorium voor Fysiologische Chemie, Rijksuniversiteit Utrecht The Netherlands

Received February 9, 1988. Revised March 29, 1988. Accepted March 29, 1988.

The decanucleotide (dc) box TNATTTGCAT is an important functional element in the K light chain gene promoter and was shown to bind nuclear proteins. We now describe the binding of nuclear factor III to dc containing oligonucleotides and define a second protein binding site in the neighbourhood of dc which turned out to be recognized by a partially purified NFIII preparation but not by highly purified NFIII (abbreviations ref. 1). This site comprises an ACCC-containing sequence element. It was further characterized by the hydroxyl radical footprinting method which is applicable to 40 bp long oligonucleotides. The significance of the ACCC-containing sequence and of the previously described 15mer sequence pd is discussed.


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