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Nucleic Acids Research, 1988, Vol. 16, No. 9 3801-3814
© 1988

Articles

Upstream sequences required for transcription of the TFIIIA gene in Xenopus oocytes

Yoshihiro Matsumoto* and Laurence Jay Korn+

Department of Genetics, Stanford University School of Medicine Stanford, CA 94305, USA

Received January 14, 1988. Revised April 7, 1988. Accepted April 7, 1988.

The DNA sequences required for efficient initiation of transcription of the Xenopus transcription factor IIIA (TFIIIA) gene were determined by microinjecting a series of deletion and linker substitution mutants into Xenopus oocyte nuclei. An upstream activating sequence, which resides between residues -283 and -238, and perhaps a second sequence between -167 and -122 preceding the transcription start site, together stimulate transcription about 30-fold. The distance between the two sequences and the distance from them to the initiation site can vary by at least 13 base pairs without loss of activity. The TFIIIA upstream sequences can stimulate transcription of other genes, for example, they stimulate transcription from the herpes thymidine kinase promoter about 30-fold.

*Present addresses: Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY 11794

+Protein Design Labs, Inc., 3181 Porter Drive, Palo Alto, CA 94304, USA


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