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Nucleic Acids Research, 1988, Vol. 16, No. 9 4053-4067
© 1988

Articles

Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication

Christopher Hatt, Michael E. Ward and Ian N. Clarke

Department of Microbiology, Faculty of Medicine (University of Southampton) South Academic Pathology Block, Southampton General Hospital Tremona Road, Southampton, SO9 4XY, UK

Received February 12, 1988. Revised March 15, 1988. Accepted March 15, 1988.

Chlamydia trachomatis serovar L1/440/LN possesses a 7498bp plasmid which was designated pLGV440. The plasmid was cloned at the BamHl site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. A fragment of the chlamydial plasmid was shown to function as a promoter in E. coli when placed upstream of the lacZ gene. The entire plasmid was sequenced by the chain termination method. Open reading frames were identified from the resulting consensus sequence together with a candidate for the plasmid origin of replication consisting of four perfect tandem repeats of a 22bp sequence, an A: T rich sequence and an open reading frame which could generate a 34.Bkdal product. The predicted polypeptide products of the open reading frames were compared by computer with all reported protein sequences. Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.


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