Skip Navigation

This Article
Right arrow Print PDF (3262K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cornelissen, M.
Right arrow Articles by Vandewiele, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cornelissen, M.
Right arrow Articles by Vandewiele, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 1 19-29
© 1989

MOLECULAR BIOLOGY

Nuclear transcriptional activity of the tobacco plastid psbA promoter

Marc Cornelissen* and Martine Vandewiele

Plant Genetic Systems NV, J.Platesustraat 22, B-9000 Gent, Belgium

* To whom correspondence should be addressed

Received October 10, 1988. Revised November 18, 1988. Accepted December 9, 1988.

The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptll coding sequences the level of expression is sufficient to obtain a selectable phenotype. The transcription start site in the nucleus is site specific and is located 4 nucleotides downstream relative to the start site used in plastids. Translational fusions of the psbA coding sequence with the nptll and bar coding sequences revealed that the psbA leader sequence and the psbA translation start codon, being the second ATG codon, are recognized by the plant cytoplasmic translation apparatus.

A promoter cassette utilisable in both E. coli and tobacco, was obtained by placing the CaMV 35S enhancer 5' to the psbA promoter.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Crop Sci.Home page
G. R. Heck, C. L. Armstrong, J. D. Astwood, C. F. Behr, J. T. Bookout, S. M. Brown, T. A. Cavato, D. L. DeBoer, M. Y. Deng, C. George, et al.
Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event
Crop Sci., January 1, 2005; 45(1): 329 - 339.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Fornara, L. Parenicova, G. Falasca, N. Pelucchi, S. Masiero, S. Ciannamea, Z. Lopez-Dee, M. M. Altamura, L. Colombo, and M. M. Kater
Functional Characterization of OsMADS18, a Member of the AP1/SQUA Subfamily of MADS Box Genes
Plant Physiology, August 1, 2004; 135(4): 2207 - 2219.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.