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Nucleic Acids Research, 1989, Vol. 17, No. 10 3655-3661
© 1989

MOLECULAR BIOLOGY

Complementation of a Dictyoslelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation

Andy C.M Chang, Keith L. Williams, Jeffrey G. Williams1 and Adriano Ceccarelli1

1Imperial Cancer Research Fund South Mimms, Herts EN6 3LD, UK School of Biological Sciences, Macquarie University Sydney. NSW 2109. Australia

Received March 3, 1989. Revised April 21, 1989. Accepted April 21, 1989.

A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D. discoideum


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