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Nucleic Acids Research, 1989, Vol. 17, No. 10 3689-3697
© 1989

CHEMISTRY

Solid phase synthesis of oligoribonucleotides using the l-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) group for the protection of the 2'-hydroxy functions and the H-phosphonate approach

Osamu Sakatsume, Michiya Ohtsuki, Hiroshi Takaku*, and Colin B. Reese1

1Department of Chemistry, King's College London Strand, London WC2R 2LS, UK Department of Industrial Chemistry, Chiba Institute of Technology Narashino, Chiba 275, Japan

*To whom correspondence should be addressed

Received February 17, 1989. Revised April 18, 1989. Accepted April 18, 1989.

The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the l-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(l,l,l,3,3,3-hexafluoro-2-propyl) phosphite for the preparation of nucleoside H-phosphonate units is also discussed in detail.


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