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Nucleic Acids Research, 1989, Vol. 17, No. 11 4293-4308
© 1989

CHEMISTRY

Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization

J.P. Albarella, R.L. Minegar, W.L. Patterson, N. Dattagupta1 and E. Carlson1

1Molecular Diagnostics Inc., Miles Research Center West Haven, CT 06516, USA Miles Inc., Elkhart, IN 46515

Received February 16, 1989. Revised May 12, 1989. Accepted May 12, 1989.

We describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. These compounds were used to label nucleic acids in the presence of lysed cell constituents. The resulting labelled nucleic acids show hybridization to a genus specific probe for E. coli.

The relative comparison of sensitivity indicates that a polyamine linker is better than a polyethylene oxide linker between the biotin and angelicin moieties.


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