Skip Navigation

This Article
Right arrow Print PDF (5743K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Middleton, K. M
Right arrow Articles by Morgan, G. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Middleton, K. M
Right arrow Articles by Morgan, G. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 13 5041-5056
© 1989

MOLECULAR BIOLOGY

An oocyte-expressed {alpha}-Dublin gene in Xenopus laevis, sequences required for the initiation of transcription

Kim M Middleton and Garry T. Morgan

Department of Genetics, University of Nottingham, Queens Medical Centre Nottingham NG7 2UH, UK

Received April 14, 1989. Revised June 7, 1989. Accepted June 7, 1989.

We have studied the expression of X{alpha}T14, a member of the {alpha}-tubulin multigene family in Xenopus laevis. Small amounts of X{alpha}T14 RNA are detectable in a range of cell types, but much higher levels are present in ovary and tissue culture cells. In oocytes X{alpha}T14 transcripts accumulate during early vitellogenesis but their level declines in more advanced stages. Faithful and efficient initiation of transcription occurred on cloned X{alpha}T14 injected into oocytes even at low template levels. We have examined the amount of transcript produced by various deletion mutants relative to a co-injected control gene. The presence of 20Obp of DNA 5' and 53bp of DNA 3' to the initiation site sufficed for high levels of promoter activity, although maximum activity required 560 bp of 5' flanking DNA. The DNA between -200 and -60 was necessary for transcription in oocytes and contains several sequence motifs implicated in transcriptional regulation including three CCAAT boxes and a sequence resembling a heat shock element. An 8 bp deletion that removed the latter element from 5kb of 5'-flanking DNA reduced promoter activity by 60%.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. K. Parker and H. W. Detrich III
Evolution, Organization, and Expression of alpha -Tubulin Genes in the Antarctic Fish Notothenia coriiceps. ADAPTIVE EXPANSION OF A GENE FAMILY BY RECENT GENE DUPLICATION, INVERSION, AND DIVERGENCE
J. Biol. Chem., December 18, 1998; 273(51): 34358 - 34369.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.