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Nucleic Acids Research, 1989, Vol. 17, No. 13 5307-5322
© 1989

MOLECULAR BIOLOGY

An enhancer associated with the mouse immunoglobin {lambda}f gene is specific for {lambda} light chain producing cells

Le thi Bich-Thuy1,2 and Cary Queen1,*

1Laboratory of Biochemistry,National Cancer Institute Bethesda,MD 20892,USA 2Laboratory of Molecular Immunology,Department of Molecular and Cellular Biology Ecole Normale Superieure de Lyon,46 Allee d'Italie,69364 Lyon,France

Received March 15, 1989. Revised May 30, 1989. Accepted May 30, 1989.

We have developed a system to study transcriptional regulation of the {lambda} immunoglobulin gene in a natural setting {lambda} light chain producing lymphoid cells. This assay system has allowed the detection of an enhancer element located 3' of the {lambda} gene coding sequence. The enhancer can stimulatetranscription from the {lambda} promoter as well as from other imnunoglobulin and unrelated promoters. Like all enhancers, the {lambda} enhancer can function in either orientation with respect to a promoter, but it is significantly more active in one orientation than in the other. The {lambda} enhancer is unusual in spanning at least 4000 bp of DNA sequence and containing several distinct subelements that haveindependent enhancer activity. The enhancer is also remarkable because it functions in {lambda} light chain producing cells but not in K chain producing cells. This fact can be interpreted to support a model of immunoglobulin gene rearrangement In which rearrangement follows and depends on transcriptional activation.

*Present address:Protein Design Labs,3181 Porter Drive,Palo Alto,CA 94300,USA


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Genes & Dev., June 1, 1990; 4(6): 978 - 992.
[Abstract] [PDF]


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