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Nucleic Acids Research, 1989, Vol. 17, No. 15 6103-6108
© 1989

MOLECULAR BIOLOGY

Chromosomal localization of nucleic acid-binding proteins by affinity mapping: assignment of the IRE-binding protein gene to human chromosome 9

Matthias W. Hentze, Hector N. Seuanez1, Stephen J. O'Brien1, Joe B. Harford and Richard D. Klausner

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD 20892 1Laboratory of Viral Carcinogenesis, National Cancer Institute, National Institutes of Health Frederick, MD 21701, USA

Received April 7, 1989. Revised July 7, 1989. Accepted July 7, 1989.

Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on nucleic acid/protein interaction may allow determination of the chromosomal localization of other RNA- or DNA-binding proteins.


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R. Klausner and J. Harford
cis-trans models for post-transcriptional gene regulation
Science, November 17, 1989; 246(4932): 870 - 872.
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