Nucleic Acids Research, 1989, Vol. 17, No. 16 6485-6496
© 1989
MOLECULAR BIOLOGY |
Efficient initiation of mammalian mRNA translation at a CUG codon
Department of Biochemistry, University of Cambridge Cambridge CB2 1QW, UK
*To whome correspondence should be addressed
Received June 23, 1989. Accepted July 21, 1989.
Nucleotide substitutions were made at the initiation codon of an influenza virus NS cDNA clone in a vector carrying the bacteriophage T7 promoter. When capped mRNA transcripts of these constructs were translated in the rabbit reticulocyte lysate, a change in the initiation codon from ... AUAAUGG... to ...AUACUGG... reduced the in vitro translational efficiency by only 50–60%, and resulted in only a small increase in the yield of short products presumed to be initiated at downstream sites. Synthesis of the full-length product was initialed exclusively at the mutated codon, with negligible use either of in-frame upstream CUG or GUG codons, or of an in-frame downstream GUG codon. We conclude that CUG has the potential to function as an efficient initiation codon in mammalian systems, at least in certain contexts.
+ Present address: Department of Biology, University of California at San Diego, La Jolla, CA 92093, USA
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