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Nucleic Acids Research, 1989, Vol. 17, No. 17 6959-6967
© 1989

MOLECULAR BIOLOGY

Forks and combs and DNA: the synthesis of branched oligodeoxyribonucleotides

Thomas Horn and Mickey S. Urdea*

Chiron Corporation 4560 Horton Street, Emeryville, CA 94608, USA

*To whom correspondence should be addressed

Received May 15, 1989. Accepted June 28, 1989.

Nucleoside phosphoramidite derivatives containing two protected primary hydroxyl functions have been incorporated into synthetic oligonucleotides as ‘branching monomers’. With selective deprotection, multiple identical copies of an additional oligonucleotide can be incorporated to form fork- or comb- like structures for use as signal amplification materials in nucleic acid hybridization assays.


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