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Nucleic Acids Research, 1989, Vol. 17, No. 18 7403-7415
© 1989

MOLECULAR BIOLOGY

Immunoglobulin x light chain gene promoter and enhancer are not responsible for B-cell restricted gene rearrangement

Michele Goodhardt, Charles Babinet1, Georges Lutfalla, Sacha Kallenbach, Patricia Caveher and Francois Rougeon

1Unité de Généetique des Mammiferes, LA CNRS 361, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France Unité de Généetique Biochimie du Développement

Received July 14, 1989. Revised August 23, 1989. Accepted August 23, 1989.

We have produced transgenic mice which synthesize chimeric mouse-rabbit immunoglobulin (Ig) x light chains following in vivo recombination of an injected unrearranged k gene. The exogenous gene construct contained a mouse germ-line k variable (Vk) gene segment, the mouse germ-line joining (Jx) locus including the enhancer, and the rabbit b9 constant (Ck) region. A high level of V-J recombination of the x transgene was observed in spleen of the transgenic mice. Surprisingly, a particularly high degree of variability in the exact site of recombination and the presence of non germ-line encoded nucleotides (N-regions) were found at the V-J junction of the rearranged x transgene. Furthermore, unlike endogenous x genes, rearrangement of the exogenous gene occurred in T-cells of the transgenic mice. These results show that additional sequences, other than the heptamer-nonamer signal sequences and the promoter and enhancer elements, are required to obtain stage and lineage specific regulation of Ig x light chain gene rearrangement in vivo


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