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Nucleic Acids Research, 1989, Vol. 17, No. 19 7723-7734
© 1989

MOLECULAR BIOLOGY

Characterization of a rat myosin alkali light chain gene expressed in ventricular and slow twitch skeletal muscles

Muthu Periasamy*,, Raj Wadgaonkar1, Chandra Kumar2, Barbara Jill Martin and M A.Q. Siddiqui1

Department of Physiology and Biophysics, University of Vermont College of Medicine Given Medical Building, Burlington, VT 05405 1Department of Anatomy and Cell Biology, State University of New York Brooklyn, NY 11203 2Department of Tumor Biology, Schering Corporation 60 Orange Street, Bloomfield, NJ 07003, USA

*To whom correspondence should be addressed

Received June 30, 1989. Revised August 25, 1989. Accepted August 25, 1989.

Mammalian cardiac muscle contains two myosin alkali light chains: 1) the atrial light chain (MLClA), and 2) the ventricular light chain (MLClV) predominantly expressed either in the atrium or in the ventricle. In this report we describe the isolation and characterization of the complete gene for rat MLClV. The rat MLClV gene is ~6.5 kb long and the mRNA coding sequences are organized in 7 different exons. Comparison of this gene sequence with other known MLCl gene sequences revealed that the exon-intron organization is highly conserved within the MLCl gene family. The derived protein sequence of rat MLClV showed a higher sequence homology with human ventricular (96%) MLClV than with rat fast skeletal MLClf (74%), suggesting functional similarities between different MLClV proteins. S1 nuclease mapping and primer extension analysis demonstrated that this gene is expressed only in ventricular and slow twitch skeletal muscle tissues and is transcribed from the same promoter and transcription initiation site.


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