Nucleic Acids Research, 1989, Vol. 17, No. 19 7821-7831
© 1989
MOLECULAR BIOLOGY |
Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants
Cold Spring Harbor Laboratory PO Box 100, Cold Spring Harbor, NY 11724, USA
*To whom correspondence should be addressed
Received May 6, 1989. Revised July 19, 1989. Accepted August 17, 1989.
U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript. The U6 gene of the fission yeast Schizosaccharomyces pombe is unusual in that it is interrupted by an intron whose structure is similar to those found in pre-mRNAs. As part of our previous analysis of three S. pombe temperature sensitive pre-mRNA splicing mutants we examined their spliceosomal snRNA content. In contrast to the other snRNAs, the amount of U6 RNA is reduced at the restrictive temperature in all three of the mutants compared to the wild type. To investigate the cause of this reduction we have analyzed the efficiency of splicing of the U6 RNA precursor (U6 pre-RNA) in the pre-mRNA splicing mutants. At the restrictive temperature the ratio of unspliced U6 precursor to mature RNA is elevated in the mutants compared to the wild type grown under identical conditions, indicating a defect in U6 pre-RNA splicing. In this regard, the U6 RNA precursor behaves similarly to pre-mRNAs. Unspliced U6 pre-RNA was also detected in wild type cells under certain growth conditions.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. H. Carnahan, A. Feoktistova, L. Ren, S. Niessen, J. R. Yates III, and K. L. Gould Dim1p Is Required for Efficient Splicing and Export of mRNA Encoding Lid1p, a Component of the Fission Yeast Anaphase-Promoting Complex Eukaryot. Cell, March 1, 2005; 4(3): 577 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Kaiser, J. Chi, and D. A. Brow Position-dependent function of a B block promoter element implies a specialized chromatin structure on the S.cerevisiae U6 RNA gene, SNR6 Nucleic Acids Res., August 10, 2004; 32(14): 4297 - 4305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Ohi, A. J. Link, L. Ren, J. L. Jennings, W. H. McDonald, and K. L. Gould Proteomics Analysis Reveals Stable Multiprotein Complexes in Both Fission and Budding Yeasts Containing Myb-Related Cdc5p/Cef1p, Novel Pre-mRNA Splicing Factors, and snRNAs Mol. Cell. Biol., April 1, 2002; 22(7): 2011 - 2024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhou, Y.-Q. Chen, Y.-P. Du, and L.-H. Qu The Schizosaccharomyces pombe mgU6-47 gene is required for 2'-O-methylation of U6 snRNA at A41 Nucleic Acids Res., February 15, 2002; 30(4): 894 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. McDonald, R. Ohi, N. Smelkova, D. Frendewey, and K. L. Gould Myb-Related Fission Yeast cdc5p Is a Component of a 40S snRNP-Containing Complex and Is Essential for Pre-mRNA Splicing Mol. Cell. Biol., August 1, 1999; 19(8): 5352 - 5362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Tani and Y Ohshima mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing. Genes & Dev., June 1, 1991; 5(6): 1022 - 1031. [Abstract] [PDF]
|
|