Skip Navigation

This Article
Right arrow Print PDF (4088K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Henninghausen, L.
Right arrow Articles by Pittius, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henninghausen, L.
Right arrow Articles by Pittius, C. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 20 8197-8206
© 1989

MOLECULAR BIOLOGY

xB elements strongly activate gene expression in non-lymphoid cells and function synergistically with NF1 elements

Lothar Henninghausen*, Priscilla A. Furth and Chirstoph W. Pittius+

Laboratory of Biochemistry and Metabolism, National Institutes of Health Building 10 Room 9N113, Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received June 8, 1989. Revised September 14, 1989. Accepted September 14, 1989.

xB elements have been described as lymphoid-specific transcriptional activators. Here we show that xB elements are able to stimulate expression from test promoters more than 100-fold in T47D and 3T3 non-lymphoid cells. We also demonstrate that nuclear proteins from T47D cells form two prominent complexes with HIV x B sites. Since the complexes formed in nuclear extracts from T47D and PHA/PMA stimulated Jurkat cells comigrate in polyacrylamide gels, we suggest that the respective binding protein in T47D cells is either N-xB or a closely related family member. In addition we provide evidence that NF1 and xB elements can act synergistically to further increase transcriptional activity.

+Present address: Holland Research Laboratories, American Red Cross, Rockville, MD 20855, USA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S.-J. Chou, K.-N. Lai, and B.-c. Chung
Characterization of the Upstream Sequence of the Human CYP11A1 Gene for Cell Type-specific Expression
J. Biol. Chem., September 6, 1996; 271(36): 22125 - 22129.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.