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Nucleic Acids Research, 1989, Vol. 17, No. 21 8463-8474
© 1989

MOLECULAR BIOLOGY

In vitro preferential topoisomerization of bent DNA

Micaela Caserta1, Andrea Amadei2, Ernesto Di Mauro1,2 and Giorgio Camilloni1

1Centro di Studio per Gli Acidi Nucleici, CNR Rome, Italy 2Dipartimento di Genetica e Biologia Molecolare, University of Rome ‘La Sapienza’ Rome, Italy

Received August 29, 1989. Revised September 25, 1989. Accepted September 25, 1989.

The steps of the topoisomerization reaction by calf thymus DNA topoisomerase I on a DNA domain containing an intrinsically bent DNA sequence have been analyzed. High preferentiality of binding, cleavage and topoisomerization on the bent segment relative to the rest of the DNA domain was observed. These studies show the importance of the local DNA conformation in the reaction of eukaryotic DNA topoisomerase I and the interest of the use of this enzyme as a tool for the analysis of DNA conformation and DNA dynamics.


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