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Nucleic Acids Research, 1989, Vol. 17, No. 22 9417-9423
© 1989

CHEMISTRY

Energetics of the B–H transition in supercoiled DNA carrying d(CT)x.d(AG)x and d(C)n.d(G)n inserts

V.I. Lyamichev, S.M. Mirkin, V.P. Kumarev1, L.V. Baranova1, A.V. Vologodskii and M.D. Frank-Kamenestskii

Institute of Molecular Genetics, USSR Academy of Sciences Moscow 123182, USSR 1Institute of Limnoligy, Siberian Branch of the USSR Academy of Sciences Irkutsk, USSR

Received August 25, 1989. Accepted October 12, 1989.

We have studied the B–H transition in the d(AG)x inserts of varying length under superhelical stress. The new data and previously published results for the d(G)31 insert are treated within a phenomenological model of the B–H transition, making it possible to obtain, for the first time, the energy parameters of the B–H transition in the d(AG)x and d(G)n sequences.


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