Skip Navigation

This Article
Right arrow Print PDF (821K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wang, C.-C.
Right arrow Articles by Spremulli, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, C.-C.
Right arrow Articles by Spremulli, L. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 23 9735-9747
© 1989

MOLECULAR BIOLOGY

Initiation complex formation on Euglena chloroplast 30S subunits in the presence of natural mRNAs

Chia-Chang Wang, Wesley B. Roney1, Richard L. Alston1 and Linda L. Spremulli1

Department of Biology, University of North Carolina Chapel Hill, NC 27599, USA 1Department of Chemistry, University of North Carolina Chapel Hill, NC 27599, USA

Received August 10, 1989. Revised October 30, 1989. Accepted October 30, 1989.

An in vitro system has been developed that allows the formation of translation initiation complexes with Euglena chloroplast 30S ribosomal subunits and natural mRNAs. For these experiments two regions of the Euglena chioroplast genome have been cloned behind the T7 transcriptional promotor and the corresponding RNAs synthesized in vitro. These mRNAs are capable of forming initiation complexes with chloroplast 30S subunits in the presence of fMet-tRNA and E. coli initiation factors. Deletion of the normal translation start site results in a message that is no longer recognized by the chloroplast subunits suggesting that the correct AUG initiation codon on the mRNA is being selected by the small ribosomal subunit. Initiation complex formation with the chloroplast 30S subunits is specific for chloroplast mRNAs and mRNA from the phage MS2 is not active in this system.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. Bhargava and L. L. Spremulli
Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3
Nucleic Acids Res., December 9, 2005; 33(22): 7011 - 7018.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N.-J. Yu and L. L. Spremulli
Regulation of the Activity of Chloroplast Translational Initiation Factor 3 by NH2- and COOH-Terminal Extensions
J. Biol. Chem., February 13, 1998; 273(7): 3871 - 3877.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.