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Nucleic Acids Research, 1989, Vol. 17, No. 24 10367-10372
© 1989

Articles

A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan

Yoshihiro Kubo+, Kenji Takeuchi, Sumalee Boonmar§, Tohru Katayama1, Qui-Lim Choo2, George Kuo2, Amy J. Weiner2, Daniel W. Bradley3, Michael Houghton2, Izumu Saito and Tatsuo Miyamura*

Department of Enteroviruses, National Institute of Health, 2-10-35, Kamiosaki, Shinagawa-ku, Tokyo 141 1Department of Surgery, National Tokyo Chest Hospital 3-1-1, Takegaoka, Kiyose-shi, Tokyo 204, Japan 2Chiron Corporation 4560 Horton Street, Emeryville, CA 94608 3Centers for Disease Control 1600 Clifton Road NE, Atlanta, GA 30333, USA

*To whom correspondence should be addressed

Received October 10, 1989. Revised November 13, 1989. Accepted November 13, 1989.

Recently, a cDNA from the hepatitis C virus (HCV) RNA genome has been 1solated in the USA from a chronicallyinfected chimpanzee. In order to isolate HCV cDNA derived from human material, RNA was extracted from plasma of a Japanese blood donor implicated in post-transfusion non-A, non-B hepatitis and HCV cDNA was synthesized and amplified by the PCR method using HCV-specific oligonucleotide primers. The cDNA fragment, 583 nucleotides long, showed 79.8% homology at the nucleotide level and 92.2% homology at the amino acid level compared with the prototype HCV cDNA. These results provides further evidence to show that HCV is closely assoclated with the development of post transfusion non-A, non-B hepatitis.

+Present address: Department of Neurophysiology, Tokyo Metropolitan Institute for Neurosciences, 2-6, Musashidai, Fuchu-shi, Tokyo 183, Japan

§On leave from Department of Medical sciences, Viruses Research Institute, National Institute of Health, Nonthaburi 11000, Thailand


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