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Nucleic Acids Research, 1989, Vol. 17, No. 24 10439-10454
© 1989

Articles

A 6-fold difference in the half-life of immunoglobulin µ heavy chain mRNA in cell lines representing two stages of B cell differentiation

Angela Cox and J. Spencer Emtage

Celltech Ltd 216 Bath Road, Slough SL1 4EN, Berkshire UK

Received September 13, 1989. Revised November 10, 1989. Accepted November 10, 1989.

When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of µ heavy chain mRNA increases during B cell differentiation, by measuring the rates of decay of the same endogenous µ gene in two cell lines representing the B celland the plasma cell. Using a pulse-chase protocol, it was found that there was a significant increase in the half-life of µ raRNA between the B cell and the plasma cell, and no detectable difference in the average half-life of total poly(A)+ RNA in the two cell lines. The reduced rate of decay of µ mRNA in the more differentiated cell type is almost sufficient to account for the difference in steady state µ mRNA levels between the two cell lines.


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