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Nucleic Acids Research, 1989, Vol. 17, No. 3 845-851
© 1989

MOLECULAR BIOLOGY

Structural and functional exchangeability of 5 S RNA species from the eubacterium E.coli and the thermoacidophilic archaebacterium Sulfolobus solfataricus

Joaquin Teixidò, Sergio Altamura1, Paola Londei1,* and Ricardo Amils

Centro de Biologia Molecular, UAM-CSIC Cantoblanco, Madrid, Spain 1Dipartimento Biopatologia Umana, Sezione Biologia Cellulare, Università di Roma Policlinico, Viale Regina Elena 324, Rome, Italy

*To whom correspondence should be addressed

Received October 10, 1988. Revised January 12, 1989. Accepted January 12, 1989.

The role of 5 S RNA within the large ribosomal subunit of the extremely thermophilic archaebacterium Sulfolobus solfataricus has been analysed by means of in vitro reconstitution procedures. It is shown that Sulfolobus 50 S subunits reconstituted in the absence of 5 S RNA are inactive in protein synthesis and lack 2–3 ribosomal proteins. Furthermore, it has been determined that in the course of the in vitro assembly process Sulfolobus 5 S RNA can be replaced by the correspondent RNA species of E.coli; Sulfolobus reconstituted particles containing the eubacterial 5 S molecule are stable and active in polypeptide synthesis at high temperatures.


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